Journal: Journal of Tissue Engineering

Article Title: Induced pluripotent stem cell-based organ-on-a-chip as personalized drug screening tools: A focus on neurodegenerative disorders

doi: 10.1177/20417314221095339

Figure Lengend Snippet: Graphic representation of the cell culture models hosted in the organs-on-a-chip involved in ADME. The scheme highlights the main cell types that should be included into the most advanced state-of-the art microfluidic devices for drug screening. The solid arrows point out the direction of diffusion of drug and/or its metabolite. The dashed arrows indicate the fate of orally administered drug in the human body. Created with BioRender.com .

Article Snippet: iPSC-derived neuron , Alzheimer’s disease , Full-length human Tau monomers and oligomers , Microfluidic standard neuronal device (Xona Microfluidics) , Localization through fluorescence microscopy , Usenovic et al. .

Techniques: Cell Culture, Drug discovery, Diffusion-based Assay

Journal: Journal of Tissue Engineering

Article Title: Induced pluripotent stem cell-based organ-on-a-chip as personalized drug screening tools: A focus on neurodegenerative disorders

doi: 10.1177/20417314221095339

Figure Lengend Snippet: iPSCs based BBB- and brain-OoC developed for drug screening.

Article Snippet: iPSC-derived neuron , Alzheimer’s disease , Full-length human Tau monomers and oligomers , Microfluidic standard neuronal device (Xona Microfluidics) , Localization through fluorescence microscopy , Usenovic et al. .

Techniques: Drug Transport Assay, Permeability, Enzyme-linked Immunosorbent Assay, Confocal Microscopy, Fluorescence, Electric Cell-substrate Impedance Sensing, Immunofluorescence, Membrane, Liquid Chromatography, Chromatography, Mass Spectrometry, Proximity Ligation Assay, Microscopy

Journal: Journal of Tissue Engineering

Article Title: Induced pluripotent stem cell-based organ-on-a-chip as personalized drug screening tools: A focus on neurodegenerative disorders

doi: 10.1177/20417314221095339

Figure Lengend Snippet: Example of different organ-on-a-chip (OoC) design for the Blood-Brain-Barrier (BBB) and the brain: (a) a pumpless 2D microfluidic device mimicking the BBB and hosting a coculture of iBMECs and rat primary astrocytes, (b) a 3D BBB-on-a-chip, with iPSC-derived ECs and human primary astrocytes and pericytes embedded in a fibrin hydrogel in a microfluidic system, (c) a 2D microfluidic device hosting iPSC-derived dopaminergic, glutamatergic and GABAergic neurons in different chambers connected by microgrooves enabling connectivity studies, (d) NVU-on-a-chip microfluidic device with the BBB constituted by primary human BMVECs, astrocytes, and pericytes and the brain by iPSC-derived neurons and astrocytes embedded in a 3D collagen gel, and (e) a schematic example of an iPSC-derived brain organoid developed in a microfluidic device, embedded in a 3D Matrigel matrix. Created with BioRender.com . Source: Adapted with permission from Wang et al. in Biotechnol. Bioeng., Lee et al. in Adv. Healthc. Mater., Fantuzzo et al. in Technology, Brown et al. in J. Neuroinflammation, Wang et al. in Lab Chip. iBMECs: induced brain endothelial cells; BBB: blood-brain-barrier; iPSCs: induced pluripotent stem cells; ECs: endothelial cells; GABA: gamma-Aminobutyric acid; NVU: neurovascular unit; BMVEC: brain microvasculature endothelial cells.

Article Snippet: iPSC-derived neuron , Alzheimer’s disease , Full-length human Tau monomers and oligomers , Microfluidic standard neuronal device (Xona Microfluidics) , Localization through fluorescence microscopy , Usenovic et al. .

Techniques: Derivative Assay